Figure 11.
Posttranslational processing and stability of the AE1-4 mutants. MDCK cells stably expressing AE1-4Δ37, AE1-4Δ53, AE1-4(Y47A), or AE1-4(Y44A,Y47A) were pulsed with 35S-Translabel™ for 15 min (lanes 1 and 2), or chased for 1 h (lanes 3 and 4), or 4 h (lanes 5 and 6). At each time point, AE1 immunoprecipitates were prepared from total cell lysates using antibodies directed against the cytoplasmic domain of AE1-4. Immunoprecipitates were either undigested (lanes 1, 3, and 5) or digested (lanes 2, 4, and 6) with endo H before analysis on a 6% SDS polyacrylamide gel. Labeled anion exchangers were detected by fluorography.