Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Dec 13;147(6):1237–1248. doi: 10.1083/jcb.147.6.1237

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

AE1-4 colocalizes with phalloidin-stained microfilaments in transfected MDCK cells. MDCK cells stably expressing the AE1-4 anion exchanger were grown in subconfluent monolayers. Cells were either untreated (A, C, and E) or extracted with 0.5% Triton X-100 in PBS (B, D, and F) before fixation in 1% paraformaldehyde in PBS. The cells were then incubated with an AE1-specific peptide antibody, followed by incubation with DAR-IgG conjugated to lissamine and FITC-conjugated phalloidin. Images showing the distribution of AE1-4 (A and B) and phalloidin-stained microfilaments (C and D) were collected on a Zeiss Axiophot microscope. The merged images (E and F) were generated in Adobe Photoshop.