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. 1999 Dec 13;147(6):1237–1248. doi: 10.1083/jcb.147.6.1237

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of AE1-4 mutants in confluent MDCK monolayers. The amino acid sequence of the NH₂-terminal truncation and point mutants of AE1-4 are illustrated in A. Dots indicate sequence that is identical among all of the constructs. Point mutants are underlined. MDCK cells stably expressing AE1-4Δ21 (B), AE1-4Δ37 (C), AE1-4Δ53 (D), AE1-4(Y44A) (E), AE1-4(Y47A) (F), or AE1-4(Y44A,Y47A) (G) were grown in confluent monolayers on coverslips. The cells were fixed and incubated with an AE1-specific peptide antibody, followed by incubation with DAR-IgG conjugated to lissamine. Immunoreactive polypeptides were visualized on a Zeiss Axiophot microscope. Images were collected near the center (B, C, E, and F) or at the apical surface (D and G) of the cells.

Localization of AE1-4 mutants in confluent MDCK monolayers. The amino acid sequence of the NH₂-terminal truncation and point mutants of AE1-4 are illustrated in A. Dots indicate sequence that is identical among all of the constructs. Point mutants are underlined. MDCK cells stably expressing AE1-4Δ21 (B), AE1-4Δ37 (C), AE1-4Δ53 (D), AE1-4(Y44A) (E), AE1-4(Y47A) (F), or AE1-4(Y44A,Y47A) (G) were grown in confluent monolayers on coverslips. The cells were fixed and incubated with an AE1-specific peptide antibody, followed by incubation with DAR-IgG conjugated to lissamine. Immunoreactive polypeptides were visualized on a Zeiss Axiophot microscope. Images were collected near the center (B, C, E, and F) or at the apical surface (D and G) of the cells.