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. 1999 Dec 13;147(6):1237–1248. doi: 10.1083/jcb.147.6.1237

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of AE1-4 mutants in subconfluent MDCK monolayers. MDCK cells expressing AE1-4Δ21 (A), AE1-4Δ37 (B), AE1-4(Y44A) (C), AE1-4(Y47A) (D), or AE1-4(Y44A,47A) (E) were grown on coverslips in subconfluent monolayers. The cells were fixed and incubated with an AE1-specific peptide antibody, followed by incubation with DAR-IgG conjugated to lissamine (A–E) and FITC-conjugated phalloidin (F–J). Immunoreactive polypeptides and phalloidin stained microfilaments were visualized on a Zeiss Axiophot microscope. Images were collected at the basal surface of the cells. Merged images were generated in Adobe Photoshop (K–O). The arrows in L mark two of the actin-containing clusters that colocalize with AE1-4Δ37. The arrowheads in O indicate where AE1-4(Y44A,47A) colocalizes with cortical actin at sites of cell-cell contact.