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. 1999 Dec 13;147(6):1351–1363. doi: 10.1083/jcb.147.6.1351

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Recruitment of ZO-1/ZO-2/ZO-3 to TJs of intestinal epithelial cells in occludin-deficient mice. Frozen sections of intestinal epithelial cells in wild-type (a and b) and occludin-deficient mice (c–j) were double stained with rat anti–occludin mAb (a and c)/rabbit anti–claudin-3 pAb (b and d), rabbit anti–claudin-3 pAb (e)/mouse anti–ZO-1 mAb (f), mouse anti–ZO-1 mAb (g)/rabbit anti–ZO-2 pAb (h), or mouse anti–ZO-1 mAb (i)/rabbit anti–ZO-3 pAb (j). In occludin-deficient intestinal epithelial cells, not only ZO-1 but also ZO-2 and ZO-3 were concentrated at the claudin-3–positive TJs. Bar, 10 μm.