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Association of PDZ1 domains of ZO-1 (P1-ZO-1) with the COOH-terminal YV sequence of claudin-1 to -8 (GST-CL-1 to -8). (A) In vitro binding analyses similar to Fig. 4 were performed. P1-ZO-1 bound to all of GST-CL-1 to -8 (arrowhead). Some of the GST fusion proteins (asterisk) were partly degraded. Bar indicates a molecular mass of 31 kD. (B) COOH-terminal amino acid sequences of claudin-1 to -8. All end in YV.
