Figure 1.

drs2Δ is synthetically lethal with arf1Δ, chc1, and pan1 alleles. (A) Genetic analyses between drs2Δ and mutations that perturb the secretory pathway. Strains carrying the indicated mutations (see Materials and Methods) were crossed with a drs2Δ mutant (6210 drs2Δ or PRY6222) to generate diploids. After tetrad analyses of the progeny, the viable double mutants were streaked at 20°, 26.5°, and 37°C to compare the growth relative to parental strains carrying single mutations. *Double mutants of the alleles and drs2 that were able to grow at 20°C, where single drs2 mutants could not. ^†Double mutants that grew more slowly than either single mutant at 26.5°C. (B) Tetrad analysis of progeny derived from crossing PRY6222 (drs2Δ) with 6210 chc1-5 (chc1-5). Spores that failed to grow were predicted to be drs2Δ chc1-5 double mutants.