Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Dec 13;147(6):1223–1236. doi: 10.1083/jcb.147.6.1223

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

The drs2Δ mutant exhibits a cold-sensitive defect in the endocytic pathway. (A) Wild-type, drs2Δ, and arf1Δ strains were transformed with a plasmid carrying the Ste3-myc construct (pSL2624; Givan and Sprague 1997) and grown to early log phase in galactose to induce expression of the STE3-myc protein. Glucose was added to 3% final concentration to repress new synthesis of Ste3-myc and the cultures were shifted to 15°C. Lysates were prepared at the times indicated and immunoblotted for Ste3-myc using the 9E10 c-myc antibody. (B) Wild-type and drs2Δ cells were stained with FM4-64 on ice and shifted to 15°C to initiate endocytosis of the dye. Images were captured at 1 and 4 h after shift to 15°C.