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. 1999 Dec 13;147(6):1275–1286. doi: 10.1083/jcb.147.6.1275

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Full-length mACF7 associates with actin and MTs. COS-7 cells were transiently transfected with pFLAG-mACF7-fl and triple-labeled with rhodamine-conjugated phalloidin (A and D), mouse monoclonal anti-FLAG M2 antibody (B and E), and rat monoclonal anti–Tyr-tubulin antibody (C and F). In a small population of transfected cells, overexpressed mACF7 and endogenous MTs coaligned with some of the actin stress fibers (A–C, arrows). In most cases, colocalization of mACF7, actin, and tubulin was only observed in patches. Insets in D–F show high magnifications of squared areas that contain patches. In addition, full-length mACF7 also coaligned with MTs and partially colocalized with actin at the membrane ruffles (D and E, arrows). Bar, 20 μm.