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. 1999 Dec 13;147(6):1249–1260. doi: 10.1083/jcb.147.6.1249

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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HRP uptake stimulated by K⁺ depolarization. (a) Control. Recycled synaptic vesicles contain peroxidase reaction product. (b) TeNT (10 ng/ml for 18 h). Only occasional synaptic vesicles contain reaction product, indicating the failure of synaptic vesicles to undergo recycling. (c) BoNT A (200 ng/ml for 18 h). A large number of synaptic vesicles contain reaction product, evidence that these vesicles have been formed from surface membrane. (insets) Reaction product labeling of clathrin-coated vesicles implicates these structures in the process of vesicle recycling. Note the larger labeled structures (arrows) in a and c. That these structures are not seen in TeNT-exposed cultures suggests that they are an intermediate in the vesicle recycling pathway. Double arrowheads in b mark coated pits associated with conventional endocytosis that is unaffected by TeNT. These micrographs are the high resolution equivalents of Fig. 6, a, d, and f. Bar: (a–c) 1 μm. Insets are enlarged to 135,000×.