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Cleavage of PKN and PRK2 by apoptotic extracts from Jurkat cells treated with α-Fas mAb. In vitro translated PKN and PRK2 were incubated with apoptotic extracts (Apop. Ext.) prepared from Jurkat cells treated with 150 ng/ml α-Fas mAb for the indicated time periods. White and black arrowheads indicate uncleaved and cleaved proteins, respectively. Molecular mass markers in kDa are indicated on the left.
