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Methanol-chloroform extraction of polar lipids (Ap lipid) and protease treatment (Ap PrK) of purified A. phagocytophilum membranes from low-passage (Ap memb p7) and high-passage (Ap memb p22) bacteria removes detectable Msp2 by protein immunoblotting using MAb 20B4. Note that the typical monomeric and oligomeric Msp2 bands present without treatment are absent after treatment. mw, molecular weight (in thousands).
