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MsbA temperature-sensitive mutants isolated following Ludox density gradient enrichment of chemically mutagenized E. coli
| Mutant | Mutation | Topological locationa |
|---|---|---|
| Lud002 | S168L (TCG→TTG) | TM4, near periplasm |
| Lud131 | A270T (GCG→ACG) | TM5, near periplasm |
| Lud134 | A270T (GCG→ACG) | |
| Lud110 | T285I (ACC→ATC) | TM6, near periplasm |
Locations of mutations were determined using the SOSUI prediction program (12). TM, transmembrane domain.
