Transfer of plasmid DNA in conjugation experiments. Lanes M, BAC-Tracker supercoiled DNA ladder; 1, strain E/C/4a (donor); 2, E. coli HB101 (recipient); 3 to 5, transconjugation of E/C/4a with E. coli HB101; 6, strain E/C/5a (donor); 7, E. coli HB101 (recipient); 8 to 10, transconjugation of E/C/5a with E. coli HB101.