TABLE 2.

Effects of carbon source in aerobic culture of E. coli NZN111 on activities of key enzymes in subsequent anaerobic fermentation on glucose in flasks

Carbon source in aerobic culture	Timea	Sp act (U/mg protein) of enzymeb
		ICL	MDH	ME	PCK	PPC	PK
Glucose	Initial	0.062 ± 0.00	1.46 ± 0.09	0.070 ± 0.01	0.043 ± 0.01	0.77 ± 0.00	0.80 ± 0.07
	Final	0.060 ± 0.01	1.68 ± 0.25	0.077 ± 0.02	0.035 ± 0.01	0.70 ± 0.05	0.65 ± 0.07
Acetate	Initial	1.12 ± 0.07	5.24 ± 0.40	0.15 ± 0.02	0.93 ± 0.03	0.28 ± 0.00	0.22 ± 0.04
	Final	0.93 ± 0.07	4.85 ± 0.05	0.16 ± 0.01	0.87 ± 0.03	0.26 ± 0.01	0.18 ± 0.02

^a“Initial” represents the data obtained at the onset of anaerobic fermentation; “final” represents those obtained at the end of anaerobic fermentation.

^bData are means ± standard deviations.