TABLE 6.
Increase in numbers of CFU over time for wild-type Edwardsiella ictaluri and the EsaU mutant, 65ST, following infection of HKDM and CCO cellsa
| Cell type and time (h) postinfection | No. of CFU of wild type/well | Fold increase from time zerob | No. of CFU of 65ST/well | Fold increase from time zerob |
|---|---|---|---|---|
| HKDM | ||||
| 0 | 1,483 ± 33.3 | 616 ± 83.3 | ||
| 5 | 6,500 ± 2,179.4 | 4.3 | 517 ± 120.2** | 0.85 |
| 10 | 53,333 ± 23,154.1 | 35.3 | 817 ± 183.3*** | 1.3 |
| CCO cells | ||||
| 0 | 413 ± 48.1 | 477 ± 8.8 | ||
| 4 | 1,793 ± 218.3 | 4.3 | 283 ± 18.6*** | 0.59 |
| 8 | 5,233 ± 611.9 | 12.7 | 226 ± 21.9*** | 0.47 |
a
CCO and HKCM cells were infected in 24-well plates at MOIs of 1 CFU:10 HKDM cells and 1 CFU:1 CCO cell. CFU data are the means ± standard errors of the results for triplicate wells and are representative of one of three experiments. A significant difference for mutant compared to wild type following a log transformation of the CFU data are indicated by asterisks. **, P < 0.01; ***, P = 0.001.
b
Time zero is at 90 min postinfection (30 min of exposure and 60 min of gentamicin killing).