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Analysis of anti-DEN virus IgG antibodies in patient sera using rEDIII-T antigen-based in-house IgG ELISA. Anti-DEN virus IgG antibodies in serum panels 1 (A), 2 (B), and 3 (C) were tested in an ELISA using the rEDIII-T protein as the capture antigen. Bound IgG antibodies were detected using anti-human IgG-HRPO/TMB. The dotted line indicates the cutoff absorbance (A450 of 0.34). Acute- and convalescent-phase sera are indicated by the solid and open symbols, respectively.
