TABLE 4.
Evaluation of rEDIII-T protein as a DEN diagnostic antigen
| Antibody statusa | No. of samples tested to determine the presence ofb:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Anti-DEN virus IgM
|
Anti-DEN virus IgG
|
|||||||
| Panel 1 | Panel 2 | Panel 3 | Total | Panel 1 | Panel 2 | Panel 3 | Total | |
| Ref+ Test+ | 37 | 52 | 0 | 89 | 38 | 49 | 0 | 87 |
| Ref− Test+ | 7 | 6 | 0 | 13 | 2 | 7 | 0 | 9 |
| Ref+ Test− | 5 | 8 | 0 | 13 | 5 | 5 | 0 | 10 |
| Ref− Test− | 31 | 16 | 39 | 86 | 35 | 21 | 39 | 95 |
Ref+, antibody present as determined by the reference assay; Ref−, antibody absent as determined by the reference assay; Test+, antibody present as determined by the in-house ELISA; Test−, antibody absent as determined by the in-house ELISA. Anti-DEN virus IgM antibodies were determined using the commercial MAC-ELISA kit (Ref) and the in-house IgM ELISA (Test). Anti-DEN IgG antibodies were determined using the HI assay (Ref) and the in-house IgG ELISA (Test). The cutoff values (mean + 3 SD) used for the IgG and IgM in-house ELISAs were 0.34 and 0.54, respectively.
A total of 201 samples were tested. The number of samples in each panel was as follows: in panel 1, 80 samples; in panel 2, 82 samples; and in panel 3, 39 samples.