TABLE 2.
Adding MMLA, tryptophan, or antibodies to TGF-β does not consistently modify PPD-b-driven IFN-γ release by PBMC of cattle experimentally infected with M. bovis
| Animala | IFN-γ release driven byb:
|
||||
|---|---|---|---|---|---|
| PPD-b | PPD-b + MMLA | PPD-b + anti-TGF-β | PPD-b + tryptophan | PBS | |
| 1 | 1.56 | 1.51 | 1.88 | 1.61 | 0.05 |
| 2 | 3.18 | 3.20 | 3.07 | 3.19 | 0.06 |
| 3 | 2.86 | 2.73 | 3.03 | 2.68 | 0.09 |
| 4 | 1.48 | 1.49 | 1.90 | 1.41 | 0.06 |
| 5 | 2.10 | 1.91 | 2.49 | 1.95 | 0.06 |
| 6 | 0.40 | 0.37 | 0.51 | 0.36 | 0.05 |
| 7 | 0.76 | 0.75 | 1.06 | 0.74 | 0.11 |
| 8 | 3.66 | 3.59 | 3.20 | 3.58 | 0.14 |
| 9 | 1.85 | 1.70 | 1.77 | 1.63 | 0.07 |
| Uninfected animal Ac | 0.05 | 0.06 | 0.06 | 0.05 | 0.05 |
| Uninfected animal Bc | 0.11 | 0.10 | 0.04 | 0.12 | 0.05 |
a
Animals 1 to 9 were infected with 5 × 103 CFU of M. bovis by the intratracheal route, and blood was sampled 15 weeks after infection.
b
Results are expressed in OD units for duplicate wells.
c
Uninfected cattle used as controls.