TABLE 3.

Adding IL-2 or GM-CSF enhances IFN-γ release in response to antigens but also stimulates IFN-γ release without antigens in experimentally infected and uninfected cattle

Animala	IFN-γ release driven byb:
	PPD-b	PPD-b + GM-CSF	PPD-b + IL-2	PPD-a	PPD-a + GMCSF	PPD-a + IL-2	IL-2 only	GMCSF only	PBS
1	1.56	2.99	2.55	1.01	3.08	2.42	0.75	2.74	0.05
2	3.18	2.97	3.31	2.21	3.01	3.15	0.48	3.10	0.06
3	2.86	3.17	3.29	0.51	3.35	2.12	0.68	3.15	0.09
4	1.48	3.41	3.37	0.42	3.47	2.27	0.79	3.33	0.06
5	2.10	3.56	3.34	0.70	2.83	2.65	1.88	3.34	0.06
6	0.40	3.45	1.51	0.18	3.38	1.24	0.66	2.59	0.05
7	0.76	3.54	2.36	0.48	3.28	2.15	0.92	3.25	0.11
8	3.66	3.41	3.77	2.17	2.59	3.41	0.07	2.60	0.14
9	1.85	3.79	3.58	0.59	3.34	2.67	1.25	3.34	0.07
Uninfected animal Ac	0.05	2.28	0.13	0.06	2.45	0.15	0.14	2.45	0.05
Uninfected animal Bc	0.11	0.04	2.00	0.16	0.04	1.83	1.50	0.04	0.05

^aAnimals 1 to 9 were infected with 5 × 10³ CFU of M. bovis by the intratracheal route, and blood was sampled 15 weeks after infection.

^bResults are expressed in OD units for duplicate wells.

^cUninfected cattle used as controls.