TABLE 6.
Adding a neutralizing antibody against IL-10 enhances the ESAT-6/CFP-10-driven IFN-γ release in most experimentally infected cattle
Animala | IFN-γ release driven byb:
|
|||
---|---|---|---|---|
PBS | Anti-IL-10 only | ESAT-6/CFP-10 | ESAT-6/CFP-10 + anti-IL-10 | |
1 | 0.06 | 0.06 | 0.46 | 0.73 |
2 | 0.06 | 0.06 | 2.10 | 3.02 |
3 | 0.07 | 0.07 | 1.08 | 1.87 |
4 | 0.06 | 0.06 | 0.46 | 1.89 |
5 | 0.06 | 0.07 | 0.83 | 2.29 |
6 | 0.08 | 0.06 | 0.14 | 0.59 |
7 | 0.12 | 0.12 | 0.40 | 1.00 |
8 | 0.05 | 0.13 | 2.05 | 3.20 |
9 | 0.08 | 0.07 | 0.74 | 3.16 |
Uninfected animal Ac | 0.06 | 0.06 | 0.06 | 0.06 |
Uninfected animal Bc | 0.08 | 0.04 | 0.07 | 0.05 |
Animals 1 to 9 were infected with 5 × 103 CFU of M. bovis by the intratracheal route, and blood was sampled 15 weeks after infection.
Results are expressed in OD units for duplicate wells.
Uninfected cattle used as controls.