TABLE 1.
Primers used for RT-PCR and sequencing reactions
| Use | Forward primer
|
Reverse primer
|
||
|---|---|---|---|---|
| Designation | Sequence (5′-3′) | Designation | Sequence (5′-3′) | |
| C. fusiformis EAS gene expression | CfdmaW_1d | ACACGGATGGTATGAGATGGC | Cf_dmaW_3u | CTACGTCAGGGACAAGTCAC |
| Cf_easG_d | GTTTGCACGCACGGGAATAC | Cf_easG_u | AGGCAGTGGCAAGACTGCTG | |
| Cf_easF_d | AACGAAGGACTGGACCACTG | Cf_easF_u | GTGTTTCGCTGATCCATCGT | |
| Cf_easE_d | AAGTATATGCTCATGGTGTC | Cf_easE_u | CATATCATTGAAGTGCTGCA | |
| Cf_easD_d | TTGGCTCGCGGTACAGCTTC | Cf_easD_u | GACGATTCTCGAGGAAGACG | |
| Cf_easC_d | GTCAACTTGCCTACACATCG | Cf_easC_u | GGACCTCGTACATGCTCAAC | |
| Cf_cloA_d | AATCATGGCGTCCAGTCCAC | Cf_cloA_u | CTCTGGCTATACCAAGCTCT | |
| Cf_lpsB_d | CTGTTCACGAGAGCATCGCG | Cf_lpsB_u | CGAAACTACCATCTCAGAGC | |
| MO3 | TCAGGTCCATCGATCAGCCAGTAT | MO2 | TTTTCATCCTCTTCGCAACATTCC | |
| C. fusiformis lpsB integration test | Cf_lpsB_f | TGCGGCGAGGAACGAAGAATGG | Cf_lpsB_r | GAGACTGCGGCGGGAAGGCTAATC |
| C. fusiformis cloA integration test | Cf_cloA_f | CCTCCGAGCAGCCTTACTTTT | Cf_cloA_r | CTAGGCGCGTTGACCGACTTGT |
| HI-LF-P450-1 | CCCCTAGGACATTGATCGGAGA | HI-WT-P450-1 | AAGGGGCATTACTATAGGTTCTTTC | |
| UR450 | ATCGATCTGCCCTTCGCTTTCTCGTT | HI-RF-P450-1 | CGCTTGAACCCGCTATTACGAG | |