TABLE 2.
Primers and annealing temperatures used for gene-specific PCR amplification and sequencing of L. lactis isolates
| Gene | Forward primera | Reverse primera | Annealing temp (°C) |
|---|---|---|---|
| rpoA | 5′-ATGATYGARTTTGAAAAACC-3′ | 5′-ACHGTRTTRATDCCDGCRCG-3′ | 46 |
| atpA | 5′-TAYRTYGGKGAYGGDATYGC-3′ | 5′-CCRCGRTTHARYTTHGCYTG-3′ | 50 |
| bcaT | 5′-TTTKSHRTGCCDGTWGG-3′ | 5′-GGWCCHACTTCYGTYTC-3′ | 46 |
| pepN | 5′-ATKTCTTAYGCWGAYRTYGT-3′ | 5′-TTKCTTCAAGSMAWGSCC-3′ | 50 |
| pepX | 5′-TTTGGGTTGAAAGTCCAGT-3′ | 5′-CCAAGAAGAAATTCCAGC-3′ | 46 |
| pheS | 5′-CAYCCNGCHCGYGAYATGC-3′ | 5′-CCWARVCCRAARGCAAARCC-3′ | 50 |
| SSU rRNA | 5′-GCGGCGTGCCTAATACATGC-3′ | 5′-ATCTACGCATTTCACCGCTAC-3′ | 50 |
a
Y, R, H, D, K, Y, W, M, and N are degenerate nucleotides according to the degenerate nucleotide alphabet: R for A or G; M for A or C; Y for C or T; K for G or T; W for A or T; N for A, G, C or T; S for G or C; H for A, C or T; and D for A or G or T.