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QPCR standard curve of V. vulnificus in APW with or without oyster homogenate. QPCR was performed for dilutions of V. vulnificus seeded in triplicate samples of APW with or without addition of oyster homogenate (0.01 g or 0.10 g). CT values for APW alone or with addition of 0.01 g oyster homogenate showed linearity (R2 = 0.99) at concentrations of 1.8 to 5.8 log CFU/ml. Standard deviations of the means ranged from 0.01 to 0.60.
