TABLE 2.

Detection of V. vulnificus in artificially inoculated APW enrichment^a

Inoculum (log CFU/ml)b	% Positive samples before APW enrichment		% Positive samples after APW enrichment		Postenrichment CT by QPCR
	mCPC	QPCR melt	mCPC	QPCR melt	SYBR green I	TaqMan
0.01 g oyster homogenate
5.40	100	100	100	100	17.94 ± 0.56	16.74 ± 0.44
4.40	100	100	100	100	18.40 ± 0.40	16.97 ± 0.34
3.40	100	100	100	100	17.90 ± 0.58	17.64 ± 0.20
2.40	33	100	100	100	17.90 ± 0.56	16.83 ± 0.09
1.40	0	100	100	100	17.70 ± 0.60	18.56 ± 0.32
0.40	0	33	100	100	18.70 ± 0.21	17.86 ± 0.30
0.04	0	0	100	100	20.01 ± 2.09	19.87 ± 2.44
Uninoculated	0	0	0	0	35.38 ± 0.25	0.00
0.10 g oyster homogenate
5.40	100	100	100	100	15.84 ± 0.27	16.99 ± 0.77
4.40	100	100	100	100	16.40 ± 0.07	16.76 ± 0.10
3.40	33	100	100	100	16.29 ± 0.02	17.57 ± 0.21
2.40	0	33	100	100	17.01 ± 1.61	17.44 ± 1.41
1.40	0	0	100	100	17.93 ± 2.89	18.35 ± 1.63
0.40	0	0	100	100	15.55 ± 0.70	16.92 ± 0.23
0.04	0	0	100	100	20.72 ± 1.27	20.27 ± 1.43
Uninoculated	0	0	0	0	33.18 ± 2.43	0.00

^aDetection of V. vulnificus in APW, determined as percent positive samples from three tubes, was based either on observation of V. vulnificus typical colonies on selective agar (mCPC) or on melt peak analysis (QPCR) for pre- and postenrichment. Values are the means (±standard deviations) of duplicate experiments with identical results.

^bV. vulnificus inocula (log CFU/ml as determined by plate count) for seeding of MPN enrichment of PHP oyster homogenates (0.01 or 0.10 g).