TABLE 3.

Quantitation of bacterial populations in the terminal ileum and different regions of the large intestine using real-time PCR

Organism	Log10 16S rRNA gene copies/mg mucosal tissuee
	Terminal ileum (n = 6)			Ascending colon (n = 8)			Transverse colon (n = 8)			Descending colon (n = 4)
	Range	Mean	SD	Range	Mean	SD	Range	Mean	SD	Range	Mean	SD
Total eubacteria	7.9-9.5	9.1a,b,c	0.6	6.8-7.9	7.4a	0.4	6.9-8.0	7.3b	0.4	6.9-8.1	7.5c	0.5
Bacteroides	5.8-6.4	6.0	0.2	6.1-6.4	6.3	0.1	6.1-6.7	6.3	0.2	6.1-6.4	6.3	0.1
Bifidobacteria	4.4-4.8	4.7a	0.2	4.9-5.9	5.3a	0.4	4.4-5.4	4.9	0.3	4.7-6.2	5.2	0.7
Desulfovibrios	4.0-4.6	4.3	0.2	3.8-4.6	4.2	0.3	3.8-6.1	4.3	0.8	3.6-5.1	4.3	0.6
Lactobacilli	2.7-6.4	5.2a	1.5	2.8-5.7	4.5a,d	1.2	5.0-6.2	5.7	0.4	5.7-6.2	6.0d	0.2
Enterobacteria	4.1-7.4	5.7	1.3	5.1-6.6	5.5	0.5	4.3-6.5	5.7	0.7	4.5-6.2	5.4	0.8
Peptostreptococcus anaerobius	3.8-6.4	4.8	1.0	3.0-6.5	4.0	1.1	2.9-5.8	3.9	0.9	3.4-4.2	3.7	0.4
Clostridium clostridioforme	6.1-6.5	6.2	0.2	6.1-6.7	6.4	0.2	6.0-6.5	6.2	0.2	6.0-7.2	6.5	0.5
Clostridium butyricum	0-3.6	2.2	1.7	2.7-3.2	2.9	0.2	0-3.4	2.0	1.6	0-3.3	1.5	1.7
Eubacterium rectale	4.2-7.1	5.7	1.1	5.8-7.1	6.5d	0.4	4.2-6.4	5.4	0.8	5.0-7.4	6.0d	1.1
Faecalibacterium prausnitzii	3.7-5.6	4.7a	0.7	5.2-6.6	6.0a	0.6	3.9-6.4	5.2	0.9	4.3-7.4	6.0	1.3
Enterococcus faecalis	0-4.2	2.6a,b	2.0	4.0-4.7	4.3a	0.2	4.1-5.1	4.5b	0.3	0-4.5	3.2	2.2

^aA significant difference (P < 0.5) was found between populations in the terminal ileum and proximal colon.

^bA significant difference (P < 0.5) was found between populations in the terminal ileum and transverse colon.

^cA significant difference (P < 0.5) was found between populations in the terminal ileum and distal colon.

^dA significant difference (P < 0.5) was found between populations in the proximal and distal colon.

^eNo significant differences were found between populations in the proximal and transverse colon or between populations in the transverse and distal colon.