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. 2007 Sep 28;73(22):7138–7149. doi: 10.1128/AEM.01306-07

FIG. 4.

FIG. 4.

Electrophoretic mobility of components and assembled complexes on nondenaturing gels. Lane 1, M-C-48 alone; lane 2, M-T-9c alone; lane 3, asymmetrical cellulosome containing M-C-48 and M-T-9c; lane 4, Scaf6 alone; lane 5, 48t alone; lane 6, 9f alone; lane 7, M-48-9c alone; lane 8, mixed cellulosome containing Scaf6, 48t, 9f, and M-48-9c. In each lane, the concentrations of the indicated proteins were 10 μM, except in lane 8 where 5 μM of each component was used. Similar quality gels were obtained for all complexes used in this study.