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Distinguishing primary islet and βTC nuclear complexes binding to the Za region. The binding specificities of nuclear proteins were tested by EMSA. See Table 1 for the sequences of the probe and competitors. Nuclear extract from βTC or from primary cultured fetal rat islet cells grown in 16 mM glucose was added to the 32P-labeled probes shown. The glucose-responsive complex (ZaI) is indicated by the arrows.
