TABLE 1.

Coamplification of E. coli cells containing IC and H. pylori ureA^a

No. of H. pylori cells	CT value ± SD for the no. of E. coli cells added
	0	10	20	30	300
30,000	24.6 ± 0.72		25.9 ± 0.21	25.4 ± 0.22	25.2 ± 0.33
3,000	27.4 ± 0.11	28.5 ± 0.17	28.5 ± 0.89	27.9 ± 0.36	27.5 ± 0.08
300	31.7 ± 0.19	31.2 ± 0.13	31.9 ± 0.08	31.9 ± 0.42	31.7 ± 0.52
30	34.3 ± 0.78	34.4 ± 0.32	34.4 ± 0.35	35.3 ± 1.1	36.7 ± 0.54*
10	35.7 ± 0.29	35.2 ± 0.25	35.4 ± 0.22	37.7 ± 1.9	UD
5	36.7 ± 1.0	36.7 ± 1.02	36.2 ± 0.22	38.1 ± 1.6	UD

^a*, the amplification curve was distorted. UD, undetermined, and no C_T values were obtained. The C_T values ± standard deviations (SD) generated from the H. pylori cells (using the FAM probe) are averages of duplicate experiments with two replicates performed for each. The average %CV was less than 2%.