Expression of regulators of rugose colonial morphology in V. cholerae ΔhapR and Δcrp mutants. Strains C7258 (wild type [WT]), AJB51 (ΔhapR), WL7258 (Δcrp), and WL51 (ΔhapR Δcrp) were grown in LB medium to an OD600 of 1.5. Abundance of mRNAs encoding cytR (A), vpsT (B), and vpsR (C) was determined by qRT-PCR. Results were normalized to recA mRNA expression. Error bars indicate the standard deviations of at least three independent cultures.