TABLE 2.
Relative amounts of acyl-HSLs produced by wild-type bacteria and by heterologous hosts expressing cloned acyl-HSL synthase genesa
Source of extract | Relative concentrations of acyl-HSL signalsb
|
|||||||
---|---|---|---|---|---|---|---|---|
Hydroxy-acyl-HSLs
|
Alkanoyl-acyl-HSLs
|
|||||||
C6 | C7 | C8 | C10 | C4 | C5 | C6 | C8 | |
2-79 wild type | 61.10 | 7.10 | 23.40 | 7.50 | NDc | 0.20 | 0.20 | 0.10 |
30-84 wild type | 64.70 | 6.60 | 22.70 | 0.70 | 4.3 | 0.30 | 0.30 | ND |
E. coli(phzI2-79) | 60.40 | 1.70 | 33.80 | 3.50 | 0.10 | 0.05 | 0.20 | 0.10 |
E. coli(phzI30-84) | 60.20 | 5.50 | 29.50 | 4.00 | 0.01 | 0.20 | 0.20 | 0.10 |
1855-344(phzI30-84) | 65.40 | 0.30 | 26.10 | 6.80 | 0.20 | 0.30 | 0.70 | 0.30 |
E. coli(csaI30-84) | ND | ND | ND | ND | 97.80 | 1.70 | 0.04 | ND |
Values are the averages of two independent experiments.
Presented as percentages of the total amount of acyl-HSLs produced by the indicated strain.
ND, not detectable.