FIG. 2.
Optimal pH levels for DRP cleavage and thermal/pH stability. (A) The DRP cleavage assay was performed at various pH levels at 25°C. Filled circles, DERAPae; open circles, DERATma; open squares, DERAEco. (B) After incubation for 10 min at the indicated temperatures, the remaining activity was assayed. Filled circles, DERAPae; open circles, DERATma; open squares, DERAEco. (C) The enzyme was incubated for 30 min at 50°C in buffers of various pH levels, after which the remaining activity was assayed. Filled squares, DERAPae; open squares, DERATma; filled circles, DERAEco.