TABLE 1.
Primers used in the SNP assay to identify Brucella isolates at the species level
| Primer | Sequence |
|---|---|
| Target amplification | |
| glk up | 5′-TATGGAAMAGATCGGCGG-3′ |
| glk down | 5′-GGGCCTTGTCCTCGAAGG-3′ |
| omp25 up | 5′-ATGCGCACTCTTAAGTCTC-3′ |
| omp25 down | 5′-GCCSAGGATGTTGTCCGT-3′ |
| trpE up | 5′-GCGCGCMTGGTATGGCG-3′ |
| trpE down | 5′-CKCSCCGCCATAGGCTTC-3′ |
| Extension | |
| glk196R (glk-1344)a | 5′-CGCTAAGAATTTGYTCGCCGG-3′ |
| glk427R (glk-1557) | 5′-TTTTTTTTTTGAAAGGATGCGCACCGGGATGC-3′ |
| glk255R (glk-1403) | 5′-TTTTTTTTTTTTTTTTTTTTAGGGTGGGCGTGATCTTGTCGGC-3′ |
| omp151F (omp25-3627) | 5′-TGGCTATACCGGTCTTTACCTTGGCTA-3′ |
| omp239F (omp25-3715) | 5′-TTTTTTTTTTTTTTTTTCTTTGCTGGCTGGAACTTCCAG-3′ |
| trp290R (trpE-2858) | 5′-TTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACCTTGGCGCCCGTCTGG-3′ |
a
Primer name with designation of equivalent site in original multilocus sequencing description (32) in parentheses.