TABLE 3.
Properties of interrogation primers included in the final multiplex SNP assay
| Extension primer | Site interrogateda | Species identified | Baseb (unique/common) | Primer size (bp) | Tmc (°C) | SNP locus tag (bp) |
|---|---|---|---|---|---|---|
| glk196R | glk-1344 | B. neotomae | C/Td | 21 | 71.4 | 18.9 |
| omp151F | omp25-3627 | B. abortus | T/C | 27 | 70.1 | 26.0 |
| glk427R | glk-1557 | B. ovis | T/C | 32 | 77.1 | 30.85 |
| omp239F | omp25-3715 | B. canis | A/C | 39 | 69.0 | 38.0 |
| glk255R | glk-1403 | B. melitensis | C/G | 43 | 77.5 | 45.1 |
| trp290R | trpE-2858 | Marine mammal | T/C | 49 | 75.5 | 50.25 |
a
Designation of site interrogated in original multilocus sequencing description (32).
b
Takes into account whether the primer is in the forward or reverse orientation such that the actual base observed in fragment analysis is listed.
c
Tm of primer excluding the polynucleotide tail.
d
C shared with B. abortus ST6 isolates.