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Expression in flask and screening on a semimicro scale of the hits found with the high-throughput screening assay. The following parameters were used: enzyme dilution, 2% (vol/vol); octanal concentration, 10 mM; temperature of incubation, 20°C. Error bars refer to an average of 10 replicate experiments. Stability increase was calculated as the ratio between the differences in half-life of each variant and the wild type, divided by the half-life of the wild type, and multiplied by 100.
