FIG. 5.

Effect of several aldehydes on the stability increase of the H201S/K168I proROL mutant relative to the wild-type enzyme. Error bars refer to an average of 10 replicate experiments. The following parameters were used: initial activity, 50 U; final concentration of each aldehyde, 10 mM; time of incubation, 7 h. Stability increase was calculated as the ratio between the differences in half-life of each variant and the wild type, divided by the half-life of the wild type and multiplied by 100.