TABLE 3.

Properties of purified double mutants^a

Variant	Half-life (h)	Stability increase relative to the wild type (%)b
Wild type	10	NA
H201S/K168P	13	30
H201S/K168L	14	40
H201S/K168I	20	100
H201S/K168S	15	50
H201S/H134P	14	40

^aThe data refer to the average value from 10 replicates, with a maximum deviation among replicates of 0.002 for k_d. The octanal concentration was 10 mM; average initial activity was 50 U.

^bStability increase was calculated as the ratio between the difference in half-life of each variant and the wild type, divided by the half-life of the wild type, and multiplied by 100. NA, not applicable.