TABLE 1.

Bacterial isolates from raw milk sampled from four farms (D, F, G, and H) from April 2004 to January 2005^a

Isolate(s)	Classb	Accession no.	Closest relative in GenBank database
F8, H162, H167	α	EF204205 to -7	Brevundimonas bullata
H172	α	EF204208	Brevundimonas diminuta
G124	α	EF204209	Brevundimonas nasdae
G125	α	EF204210	Brevundimonas vesicularis
H196	β	EF204211	Janthinobacterium lividum
H208, H209, H212, H213, H214	β	EF204212 to -16	Delftia tsuruhatensis
H103, H177, H211	γ	EF204217 to -19	Stenotrophomonas maltophilia
D13, D14, D15, D16, F9, G9, G10, G11, G25, G94, G96, G101	γ	EF204220 to -31	Pseudomonas brennerii
D18	γ	EF204232	Pseudomonas fluorescens
D21, D26, D27, F1, F14, F15, F16, F17, F18, F19, F20, F21, F29, F121, D19	γ	EF204233 to -47	Pseudomonas putida
F112, F117, F127	γ	EF204248 to -50	Pseudomonas synxantha
F165	γ	EF204251	Pseudomonas veronii
F118, H202	γ	EF204252 and -3	Pseudomonas sp.
H36	γ	EF204255	Moraxella osloensis
D10, F5, G30, G13	γ	EF204256 to -9	Acinetobacter baumannii
D12, F166	γ	EF204260 and -1	Acinetobacter calcoaceticus
D25	γ	EF204262	Acinetobacter haemolyticus
F22, F24, F25, F26, F27, F146, F147, H13, H104	γ	EF204263 to -71	Acinetobacter johnsonii
D5, D6, D119, F32, G8, G14, G15, G16, G26, G29, G34, G38, H12	γ	EF204272 to -84	Acinetobacter lwoffii
H22	γ	EF204285	Psychrobacter arenosus
G28, G40, G132, H97	γ	EF204286 to -9	Psychrobacter faecalis
F162	γ	EF204254	Psychrobacter phenylpyruvica
H210	γ	EF204290	Aeromonas media
H201	γ	EF204291	Enterobacter amnigenus
F4, H200	γ	EF204292 and -3	Klebsiella oxytoca
G115, G116	γ	EF204294 and -5	Serratia fonticola
F10, F11	γ	EF204296 and -7	Serratia marcescens
F125	Bacilli	EF204298	Exiguobacterium undae
D105	Bacilli	EF204299	Kurthia gibsonii
H119	Bacilli	EF204300	Facklamia ignava
H96	Bacilli	EF204301	Staphylococcus equorum
G36	Bacilli	EF204302	Staphylococcus hominis
G138a	Bacilli	EF204303	Staphylococcus saprophyticus
G127, H92, H93	Bacilli	EF204304 to -6	Staphylococcus sciuri
H16	Bacilli	EF204307	Macrococcus caseolyticus
H20	Bacilli	EF204308	Aerococcus viridans
H181a	Bacilli	EF204309	Trichococcus pasteurii
G117 G97	Bacilli	EF204310 and -1	Carnobacterium maltaromaticum
H126a, H127	Bacilli	EF204312 and -3	Carnobacterium viridans
F122, G129, G130, G141	Bacilli	EF204314 to -17	Enterococcus faecium
F154, F155, F157, G139, H166, H180, H206	Bacilli	EF204318 to -24	Enterococcus aquimarinus
G6	Bacilli	EF204325	Leuconostoc citreum
D28, F170, G1, G2, G3, G4, G17, G18, G19, G20, G31, G41, G98, G119, G120	Bacilli	EF204326 to -35, EF204336 to -40	Leuconostoc mesenteroides
G21 G23	Bacilli	EF204367, EF204369	Leuconostoc mesenteroides subsp. mesenteroides
D102, D104, H14, H17, H18, H19, H25, H28, H31, H32	Bacilli	EF204341 to -50	Streptococcus parauberis
F7, G35, D22, D23, D24, D29, D30, F124, F65, H207	Bacilli	EF204351 to -60	Lactococcus lactis
D8, D90, D124, F150, G22, G99, G100, G105, G118, G121	Bacilli	EF204361 to -64, EF204368, EF204370 to -4	Lactococcus raffinolactis
F116, F123	Bacilli	EF204365 and -6	Lactococcus raffinolactis
H26	Actinobacteria	EF204375	Arthrobacter agilis
D4	Actinobacteria	EF204376	Arthrobacter tumbae
F30	Actinobacteria	EF204377	Arthrobacter oxydans
F164	Actinobacteria	EF204378	Arthrobacter methylotrophus
D85, D122	Actinobacteria	EF204379 and -80	Cellulosimicrobium funkei
F144, H23	Actinobacteria	EF204381 and -2	Kocuria rhizophila
H29, H7, H21	Actinobacteria	EF204383 to -5	Rothia mucilaginosa
H126b	Actinobacteria	EF204386	Corynebacterium casei
D110	Actinobacteria	EF204387	Brachybacterium sacelli
G134a	Actinobacteria	EF204388	Brachybacterium conglomeratum
G122, G133, H187	Actinobacteria	EF204389 to -91	Pseudoclavibacter helvolus
D84, D103, D118, D126, H193	Actinobacteria	EF204392 to -96	Microbacterium lacticum
D2, D3, D91, D92, D93, D96, D11, 15D127, F142, F143, F145, F151, F152, F159, F173, F175, F177, F171, G123, G135, H95, H100, H101, H102, H111, H163, H204, F109, H4, H5, H6, H173, H185, H190	Actinobacteria	EF204397 to -430	Microbacterium oxydans or Microbacterium maritypicum
D114, H110b	Actinobacteria	EF204431 and -2	Microbacterium paraoxydans
H34	Actinobacteria	EF204433	Microbacterium trichotecenolyticum
H39	Actinobacteria	EF204434	Rhodococcus corynebacterioides
H11, F2, G128, G138b, H99, H170, H181b, H182	Actinobacteria	EF204435 to -42	Rhodococcus erythropolis
D117	Actinobacteria	EF204443	Rhodococcus fascians
H198	Flavobacteria	EF204444	Flavobacterium johnsoniae
H205	Flavobacteria	EF204445	Flavobacterium frigidimaris
H9, H10, H15, H38	Flavobacteria	EF204446 to -8	Chryseobacterium hispanicum
H27, H197, H30, H8	Flavobacteria	EF204454 and -5, EF204449, EF204451	Chryseobacterium joostei
F108	Flavobacteria	EF204452	Chryseobacterium formosense
F13	Flavobacteria	EF204453	Chryseobacterium soldanellicola
H2	Flavobacteria	EF204456	Chryseobacterium piscium
G24, G32, G39	Flavobacteria	EF204457 to -9	Chryseobacterium scophthalmum
H1	Flavobacteria	EF204460	Epilithonimonas tenax
H105, H168	Sphingobacteria	EF204461 and -62	Sphingobacterium daejeonense
H183, H184	Sphingobacteria	EF204463 and -4	Sphingobacterium thalpophilum
H216	Sphingobacteria	EF204465	Sphingobacterium spiritivorum
H179	Sphingobacteria	EF204466	Sphingobacterium spiritivorum
F12	Sphingobacteria	EF204467	Flexibacter aurantiacus subsp. Excathedrus
H37, F163	Sphingobacteria	EF204468 and -9	Pedobacter aurantiacus

^aThe letters D, F, G, and H in the isolate names refer to the different farms from which the bacteria were isolated. Isolate names in boldface represent a probable new unidentified species (with<97.5% 16S rRNA gene similarities to any known species). Isolate names that are underlines and in boldface represent a probable new unidentified genus (with <93.8% 16S rRNA gene similarities to any known species). For more details, see Tables S1 to S7 in the supplemental material.

^bα, Alphaproteobacteria; β, Betaproteobacteria; γ, Gammaproteobacteria.