TABLE 2.
S. xylosus S04002-, S04009-, and 00-1747-specific sequences with significant protein matches in the GenBank database
Function group and predicted encoded protein | Fragment | E value | % Identity | Organism | Accession no. | Homology found
|
||
---|---|---|---|---|---|---|---|---|
S04002 | S04009 | 00-1747 | ||||||
Metabolism | ||||||||
Biotin metabolism | ||||||||
Biotin synthase BioB | E5 | 2.00E-44 | 85 | Staphylococcus aureus COL | ER935916 | + | ||
E129 | 2.00E-34 | 80 | S. aureus COL | ER935940 | + | |||
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA BioD | E5 | 1.00E-17 | 62 | S. aureus Mu50 | ER935916 | + | ||
D128 | 6.00E-125 | 82 | S. aureus COL | ER935891 | + | |||
D128 | 4.00E-26 | 63 | Staphylococcus epidermidis RP62A | ER935891 | + | |||
Myo-inositol catabolism | ||||||||
IolA | Mb262 | 5.00E-133 | 74 | Bacillus licheniformis ATCC 14580 | ER935857 | + | + | |
D76 | 6.00E-40 | 65 | Geobacillus kaustophilus HTA426 | ER935886 | + | |||
IolB | Mb11 | 1.00E-61 | 52 | B. licheniformis ATCC 14580 | ER935832 | + | + | |
IolC | Mb11 | 7.00E-59 | 63 | Bacillus halodurans C-125 | ER935832 | + | + | |
IolC (putative) | M141 | 1.00E-30 | 66 | B. licheniformis ATCC 14580 | ER935866 | + | + | |
IolD | M141 | 8.00E-71 | 70 | B. halodurans C-125 | ER935866 | + | + | |
IolG | E126 | 2.00E-64 | 50 | Bacillus cereus E33L | ER935938 | + | ||
IolE | Mb118 | 2.00E-79 | 63 | Clostridium tetani E88 | ER935843 | + | + | |
Pentose and glucuronate interconversion | ||||||||
d-Arabino-3-hexulose-6-phosphate formaldehyde lyase | E51 | 2.00E-14 | 95 | Staphylococcus saprophyticus ATCC 15305 | ER935923 | + | + | |
Putative hexulose-6-phosphate synthase | E28 | 4.00E-75 | 95 | S. saprophyticus ATCC 15305 | ER935918 | + | + | |
Putative 6-phospho-3-hexuloisomerase | E28 | 9.00E-77 | 83 | S. saprophyticus ATCC 15305 | ER935918 | + | + | |
UTP-glucose-1-phosphate uridyltransferase | E36 | 7.00E-75 | 90 | S. saprophyticus ATCC 15305 | ER935921 | + | ||
2-dehydro-3-deoxyphosphogluconate aldolase | Mb275 | 3.00E-78 | 63 | Bacillus thuringiensis serovar Israelensis ATCC 35646 | ER935858 | + | + | |
2-dehydro-3-deoxygluconokinase PfkB | D151 | 5.00E-63 | 55 | Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | ER935896 | + | ||
D242 | 6.00E-29 | 49 | Bacillus weihenstephanensis KBAB4 | ER935906 | + | + | ||
Alpha/beta hydrolase fold family protein | Mb59 | 3.00E-27 | 52 | Flavobacteriales bacterium HTCC2170 | ER935840 | + | ||
Fructose and mannose metabolism | ||||||||
Phosphomannomutase | E127 | 4.00E-83 | 65 | S. epidermidis RP62A | ER935939 | + | ||
Hexitol dehydrogenase | Mb27 | 4.00E-94 | 69 | S. aureus COL | ER935835 | + | + | |
M139 | 1.00E-14 | 72 | S. aureus COL | ER935864 | + | + | ||
Sorbitol dehydrogenase | M139 | 3.00E-10 | 62 | S. aureus COL | ER935864 | + | + | |
Fermentative metabolism | ||||||||
Acetoin (diacetyl) reductase ButA | E129 | 9.00E-57 | 81 | S. epidermidis ATCC 12228 | ER935940 | + | ||
α-Acetolactate synthase AlsS | E190 | 0.0 | 97 | S. saprophyticus ATCC 15305 | ER935953 | + | ||
Amino acid metabolism | ||||||||
Saccharopine dehydrogenase-related protein | D85 | 2.00E-04 | 54 | Lactococcus lactis subsp. cremoris SK11 | ER935887 | + | ||
D218 | 2.00E-35 | 64 | L. lactis subsp. cremoris SK11 | ER935905 | + | |||
Sarcosine oxidase alpha subunit | D129 | 5.00E-77 | 51 | B. thuringiensis serovar Konkukian 97-27 | ER935892 | + | ||
Sarcosine oxidase beta subunit | D298 | 4.00E-45 | 41 | B. cereus ATCC 14579 | ER935912 | + | ||
Betaine aldehyde dehydrogenase | D298 | 9.00E-25 | 63 | B. weihenstephanensis KBAB4 | ER935912 | + | ||
Dihydrodipicolinate synthase | D176 | 2.00E-06 | 42 | Rickettsia felis URRWXCal2 | ER935899 | + | ||
Other | ||||||||
Lipase | E179 | 4.00E-92 | 92 | S. saprophyticus | ER935951 | + | ||
Putative lipase | D218 | 2.00E-88 | 83 | S. saprophyticus ATCC 15305 | ER935905 | + | ||
UDP-glucose 4-epimerase | D44 | 2.00E-20 | 59 | G. kaustophilus | ER935881 | + | ||
Dihydroorotase | D23 | 1.00E-71 | 56 | B. cereus G9241 | ER935879 | + | + | |
Putative nucleoside diphosphate-sugar epimerase DTDP-D-glucose 4,6-dehydratase | D46 | 1.00E-55 | 82 | S. saprophyticus ATCC 15305 | ER935882 | + | + | |
COG1670, putative acetyltransferase | D96 | 5.00E-45 | 54 | S. saprophyticus ATCC 15305 | ER935888 | + | + | |
Aldehyde dehydrogenase AldA | D106 | 4.00E-55 | 69 | Bacillus sp. B14905 | ER935889 | + | ||
Riboflavin kinase RibR | D213 | 9.00E-10 | 40 | B. subtilis subsp. subtilis 168 | ER935904 | + | + | + |
COG2936, putative peptidase S9, prolyl oligopeptidase precursor | D273 | 5.00E-50 | 81 | S. aureus RF122 | ER935909 | + | ||
Similar to oxidoreductase | Mb127 | 8.00E-16 | 36 | S. aureus Mu50 | ER935844 | + | + | |
COG0655, putative iron-sulfur flavoprotein (hypothetical protein SAS0063) | E184 | 1.00E-16 | 53 | S. aureus MSSA476 | ER935952 | + | ||
Short-chain alcohol dehydrogenase | E139 | 9.00E-73 | 98 | S. saprophyticus ATCC 15305 | ER935942 | + | ||
COG4221, putative short-chain alcohol dehydrogenase of unknown specificity | E51 | 2.00E-42 | 100 | S. saprophyticus ATCC 15305 | ER935923 | + | ||
COG1063, alcohol dehydrogenase | M65 | 2.00E-141 | 97 | S. saprophyticus ATCC 15305 | ER935860 | + | + | |
Regulatory | ||||||||
Putative regulator of HxlAB (hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase) | E111 | 1.00E-35 | 98 | S. saprophyticus ATCC 15305 | ER935932 | + | ||
Transcriptional regulator, LysR family probable AlsR regulator | E173 | 4.00E-37 | 33 | Bacillus clausii KSM-K16 | ER935950 | + | ||
Putative regulator of sorbitol operon | D7 | 4.00E-18 | 28 | Clostridium beijerincki NCIMB 8052 | ER935875 | + | ||
Sorbitol operon activator | D254 | 2.00E-24 | 39 | C. beijerincki KSM-K16 | ER935908 | + | ||
Transcriptional regulator putative hydrogen peroxide-inducible genes activator | E85 | 3.00E-81 | 99 | S. saprophyticus ATCC 15305 | ER935928 | + | + | |
PTS multidomain regulator BglG | Mb48 | 6.00E-41 | 44 | S. aureus MSSA476 | ER935839 | + | ||
D63 | 2.00E-16 | 45 | S. aureus COL | ER935884 | + | + | ||
Putative PTS multidomain regulator EIIA putative fructose/mannitol specific | Mb147 | 6.00E-45 | 45 | S. aureus MSSA476 | ER935850 | + | + | |
PTS system enzyme II B component putative sorbitol specific | M149 | 6.00E-35 | 87 | S. aureus Mu50 | ER935867 | + | + | |
D53 | 2.00E-36 | 84 | S. aureus Mu50 | ER935883 | + | + | ||
PTS transport system IIA component putative galactitol specific | D53 | 1.00E-07 | 44 | S. aureus MRSA252 | ER935883 | + | + | |
Accessory gene regulator C agrC | E154 | 5.00E-110 | 83 | S. saprophyticus ATCC 15305 | ER935944 | + | ||
Protein SSP2020 putative ebsC transcription regulation protein | M160 | 6.00E-40 | 61 | S. saprophyticus ATCC 15305 | ER935868 | + | + | |
NmrA-like transcriptional regulator | D11 | 8.00E-22 | 38 | Salinispora tropica CNB-440 | ER935876 | + | ||
Transcriptional regulator, LysR family domain protein | E184 | 4.00E-56 | 66 | S. aureus MSSA476 | ER935952 | + | ||
E116 | 8.00E-13 | 46 | S. aureus USA300 | ER935933 | + | |||
Transcriptional regulator, GntR family/aminotransferase, class I | E3 | 1.00E-53 | 57 | S. aureus COL | ER935914 | + | + | |
Mb46 | 1.00E-49 | 59 | S. aureus COL | ER935838 | + | |||
Mb145 | 9.00E-20 | 81 | S. aureus COL | ER935849 | + | |||
COG1167, putative transcriptional regulator, GntR family | E100 | 2.00E-165 | 98 | S. saprophyticus ATCC 15305 | ER935929 | + | ||
Transcriptional regulator, AraC/XylS family | D141 | 2.00E-43 | 58 | Oceanobacillus iheyensis HTE831 | ER935895 | + | + | |
COG1733, putative transcriptional regulator | E139 | 3.00E-17 | 93 | S. saprophyticus ATCC 15305 | ER935942 | + | + | |
Cell membrane/surface structure | ||||||||
Putative autolysin (mannosyl-glycoprotein endo-β-N-acetylglucosamidase) | E73 | 3.00E-55 | 99 | S. saprophyticus ATCC 15305 | ER935926 | + | + | + |
E130 | 2.00E-178 | 97 | S. saprophyticus ATCC 15305 | ER935941 | + | |||
Autolysin (mannosyl-glycoprotein endo-β-N-acetylglucosamidase) | D294 | 5.00E-07 | 66 | S. epidermidis ATCC 12228 | ER935911 | + | + | + |
67-kDa myosin-cross-reactive streptococcal antigen-like protein | E110 | 3.00E-26 | 94 | S. epidermidis | ER935931 | + | ||
smart00255, putative Toll-interleukin receptor | D157 | 1.00E-35 | 69 | S. aureus MSSA476 | ER935897 | + | ||
Glycosyl transferase, family 2, CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase | E121 | 1.00E-32 | 40 | Clostridium phytofermentans ISDg | ER935935 | + | ||
Lipopolysaccharide modification acyltransferase | Mb127 | 8.00E-19 | 78 | S. epidermidis ATCC 1228 | ER935844 | + | + | |
Biofilm-associated protein Bap | D137 | 1.00E-18 | 28 | Staphylococcus hyicus | ER935894 | + | ||
LPXTG cell wall surface anchor family protein, hypothetical protein SSPP105 | E25 | 1.00E-27 | 98 | S. saprophyticus ATCC 15305 | ER935917 | + | ||
Cell wall surface anchor family protein, hypothetical protein SSPP105 | E34 | 2.00E-114 | 98 | S. saprophyticus ATCC 15305 | ER935920 | + | ||
Putative membrane protein of unknown function | D157 | 3.00E-05 | 36 | S. saprophyticus ATCC 15305 | ER935897 | + | ||
COG3152, predicted membrane protein | D158 | 0.056 | 31 | Staphylococcus haemolyticus | ER935898 | + | ||
COG3949, SSP0396 uncharacterized membrane protein | D294 | 9.00E-24 | 39 | S. saprophyticus ATCC 15305 | + | + | + | |
Resistance | ||||||||
Methicillin resistance protein MecA1 | Mb201 | 4.00E-38 | 52 | Staphylococcus sciuri | ER935854 | + | ||
MecR1, methicillin resistance regulatory protein | M116 | 5.00E-73 | 58 | S. aureus | ER935862 | + | ||
Metallothiol transferase FosB (fosfomycin resistance protein) | M130 | 3.00E-69 | 89 | S. haemolyticus JCSC1435 | ER935863 | + | ||
MphBM macrolide 2′-phosphotransferase II | M180 | 1.00E-92 | 86 | S. aureus | ER935869 | + | + | |
Micrococcin P1 peptide synthetase | Mb107 | 6.00E-123 | 58 | Staphylococcus equorum | ER935842 | + | + | |
Putative tetronasin resistance transmembrane protein | D178 | 3.00E-18 | 36 | B. halodurans C-125 | ER935901 | + | + | |
Aminoglycoside adenyltransferase | D121 | 7.00E-51 | 79 | S. saprophyticus ATCC 15305 | ER935890 | + | ||
Arsenate reductase | E125 | 3,00E-73 | 100 | S. xylosus plasmid Psx267 | ER935937 | + | + | + |
Arsenic efflux pump protein | E125 | 2.00E-61 | 100 | S. aureus | ER935937 | + | + | + |
Arsenic efflux pump membrane protein | D284 | 4.00E-19 | 100 | S. xylosus(Psx267) | ER935910 | + | + | + |
Arsenic resistance operon repressor | D284 | 4.00E-49 | 89 | S. aureus N315 | ER935910 | + | + | + |
Membrane protein TerC, possibly involved in tellurium resistance | D16 | 1.00E-11 | 78 | S. saprophyticus ATCC 15305 | ER935877 | + | ||
Probable drug resistance transporter of EmrB/QacA family | Mb12 | 4.00E-19 | 46 | Listeria monocytogenes 4b F2365 | ER935833 | + | + | |
Putative drug exporter of RND superfamily | E4 | 8.00E-91 | 74 | S. saprophyticus ATCC 15305 | ER935915 | + | ||
COG2329, putative antibiotic biosynthesis monooxygenase | M266 | 2.00E-27 | 55 | S. aureus JH1 | ER935873 | + | + | |
Transport | ||||||||
l-lactate permease | E117 | 2.00E-47 | 92 | S. saprophyticus ATCC 15305 | ER935934 | + | ||
Na+:myo-inositol cotransporter | Mb42 | 1,00E-56 | 70 | Bacillus clausii KSM-K16 | ER935837 | + | + | |
Amino acid permease-associated region lysine-specific permease precursor | D132 | 4.00E-58 | 53 | Pseudomonas fluorescens PfO-1 | ER935893 | + | ||
PTS enzyme IIC component putative galactitol specific I | M283 | 9.00E-34 | 57 | Salmonella enterica serovar Typhimurium LT2 | ER935874 | + | ||
Ferrous iron transport protein B | Mb142 | 8.00E-42 | 76 | S. epidermidis RP62A | ER935848 | + | + | |
ATPase copper transport | E158 | 4.00E-62 | 86 | S. aureus USA300 | ER935947 | + | ||
D182 | 4.00E-110 | 85 | S. aureus USA300 | ER935902 | + | |||
Putative permease, putative role in amino acid adenylation | E157 | 2.00E-51 | 81 | S. aureus USA300 | ER935946 | + | + | |
Putative permease of drug and metabolite transporter superfamily | E38 | 6.00E-122 | 98 | S. saprophyticus ATCC 15305 | ER935922 | + | + | |
Putative transporter, EamA family | E77 | 8.00E-50 | 53 | S. aureus Mu50 | ER935927 | + | ||
Putative ABC-type nitrate sulfonate taurine bicarbonate transport system ATPase component | M237 | 3.00E-65 | 89 | S. saprophyticus ATCC 15305 | ER935871 | + | + | |
TRAP-T family transporter, putative tripartite tricarboxylate transporter | D177 | 2.00E-17 | 49 | Rhodobacter sphaeroides 2.4.1 | ER935900 | + | + | |
ABC transporter, ATP-binding/permease protein | D19 | 6.00E-18 | 73 | S. aureus COL | ER935878 | + | ||
Putative ABC transporter permease | E61 | 1.00E-10 | 89 | S. saprophyticus ATCC 15305 | ER935925 | + | ||
Major facilitator family transporter | E142 | 3.00E-32 | 54 | Listeria innocua Clip11262 | ER935943 | + | + | |
Putative permease of the major facilitator superfamily | D301 | 4.00E-20 | 96 | S. saprophyticus ATCC 15305 | ER935913 | + | ||
Phage-related and IS elements | ||||||||
IS605/IS200-like transposase | Mb168 | 3.00E-86 | 89 | S. epidermidis ATCC 1228 | ER935851 | + | + | |
Recombinase Sin | E110 | 3.00E-75 | 94 | S. epidermidis ATCC 12228 | ER935931 | + | ||
Integrase | Mb194 | 7.00E-66 | 64 | S. aureus RF122 | ER935852 | + | ||
Phage infection protein | E168 | 0.0 | 99 | S. epidermidis RP62A | ER935949 | + | + | |
Phage terminase large subunit, 77ORF003 | Mb33 | 5.00E-68 | 62 | Bacteriophage 77 | ER935836 | + | ||
ORF001 | Mb66 | 3.00E-11 | 60 | Bacteriophage 2638A | ER935841 | + | ||
ORF094 | Mb66 | 7.00E-13 | 79 | Bacteriophage 2638A | ER935841 | + | ||
ORF028 | Mb66 | 3.00E-06 | 77 | Bacteriophage 2638A | ER935841 | + | ||
Conserved phage protein probable ORF029 of bacteriophage 37 | Mb131 | 1.00E-16 | 56 | S. haemolyticus JCSC1435 | ER935845 | + | ||
phiPVL ORF050-like protein | Mb133 | 3.00E-06 | 36 | S. aureus USA300 | ER935846 | + | ||
ORF025 | Mb137 | 3.00E-08 | 51 | Bacteriophage EW | ER935847 | + | ||
ORF60 | Mb215 | 2.00E-06 | 46 | S. phage 187 | ER935856 | + | ||
ORF077 | Mb215 | 0.012 | 41 | Bacteriophage EW | ER935856 | + | ||
Capsid protein, 77ORF006 | M85 | 7.00E-55 | 80 | Bacteriophage 77 | ER935861 | + | ||
77ORF042 | M85 | 1.00E-12 | 47 | Bacteriophage 77 | ER935861 | + | ||
ORF001 | M256 | 3.00E-59 | 59 | Bacteriophage 2638A | ER935872 | + | ||
Phage helicase, C terminal | M200 | 2.00E-22 | 31 | Clostridium thermocellum ATCC 27405 | ER935870 | + | ||
Integrase, ORF010 | D65 | 2.00E-30 | 56 | Bacteriophage 52A | ER935885 | + | ||
Hypothetical protein SSPP103, phage infection protein | E160 | 3.00E-11 | S. saprophyticus ATCC 15305 | ER935948 | + | + | + | |
Hypothetical protein SAR0381, probable phage protein | Mb279 | 5.00E-09 | 38 | S. aureus MRSA252 | ER935859 | + | ||
Unknown function | ||||||||
COG3877, unnamed protein product | E31 | 7.00E-37 | 79 | S. haemolyticus JCSC1435 | ER935919 | + | ||
Hypothetical protein SSP0169 | E55 | 2.00E-09 | 85 | S. saprophyticus ATCC 15305 | ER935924 | + | ||
Similar to unknown protein SH2317 | E108 | 7.00E-09 | 60 | S. haemolyticus | ER935930 | + | ||
Hypothetical protein SE2200 | E123 | 3.00E-09 | 89 | S. epidermidis ATCC 12228 | ER935936 | + | + | |
Hypothetical protein SH0055 | E156 | 1.00E-34 | 70 | S. haemolyticus JCSC1435 | ER935945 | + | ||
COG1284, uncharacterized conserved protein SSP1949 | Mb26 | 2.00E-36 | 84 | S. saprophyticus ATCC 15305 | ER935834 | + | + | |
Hypothetical protein cn33 | Mb198 | 2.00E-19 | 37 | S. epidermidis bacteriophage CNPH82 | ER935853 | + | ||
Hypothetical protein SH1793 | Mb215 | 0.001 | 54 | S. haemolyticus JCSC1435 | ER935856 | + | ||
Hypothetical protein, no similarity found | Mb213 | ER935855 | + | |||||
Hypothetical protein SAB0188 | M139 | 7.00E-07 | 64 | S. aureus RF122 | ER935864 | + | + | |
Hypothetical protein Mbar_A2529 | M266 | 2.00E-12 | 30 | Methanosarcina barkeri strain Fusaro | ER935873 | + | + | |
Hypothetical protein | D39 | 8.00E-12 | 45 | S. aureus Mu50 | ER935880 | + | ||
Truncated hypothetical protein | D121 | 1.00E-10 | 83 | S. saprophyticus ATCC 15305 | ER935890 | + | ||
Hypothetical protein SSP2368 | D197 | 4.00E-05 | 49 | S. saprophyticus ATCC 15305 | ER935903 | + | + | |
No significant similarity found | M140 | ER935865 | + | |||||
No significant similarity found | D246 | ER935907 | + |