FIG. 3.

Western spot immunodetection of A. caldus TetH. Lane 1, A. caldus cells grown in mixed culture with “F. acidarmanus” on pyrite; lane 2, negative control with “F. acidarmanus” grown on pyrite; lane 3, A. caldus grown on a mixture of S⁰ and tetrathionate; lane 4, A. caldus grown on S⁰; lane 5, A. caldus grown on tetrathionate; and lane 6, A. caldus grown on thiosulfate. All lanes except lane 6 (100 μg) were loaded with 50 μg total protein. The dashed line indicates where the image of the single gel was cropped.