FIG. 3.

ERG11/MDR1 chimeric luciferase reporter gene constructs. (A) The non-azole-inducible MDR1 promoter (MDR1-500) (7) was inserted upstream of the luciferase reporter gene (RLUC). The azole-inducible ERG11 region, identified in Fig. 1 and located at bp 301 to 205 upstream of the start of translation (region 301-205), was ligated upstream of the MDR1 promoter in its native, forward orientation (FOR). It also was ligated upstream of the MDR1 promoter in the reverse orientation (REV). (B) ERG11/MDR1 chimeric and reverse chimeric strains were grown in the presence and absence of 10 μg ml⁻¹ MICA for 48 h, and luciferase assays were performed. Induction (n-fold) is expressed as the ratio of luciferase activity in the presence of drug to the luciferase activity in the absence of drug for each construct. These results are from one representative experiment (see Materials and Methods).