TABLE 1.
Strains used in this study
| Designation | Archive no. | Parental strain | Relevant genotypea | Reference or source |
|---|---|---|---|---|
| SC5314 | TW00607 | Clinical isolate | Wild type | 6 |
| CAI8 | TW09007 | SC5314 | ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 | 4 |
| MDR1 | TW10072 | CAI8 | ADE2::(proMDR1[500]-RLUC)/ade2::hisG | 7 |
| FOR | TW13338 | CAI8 | ADE2::(proERG11[301-205] proMDR1[500]-RLUC)/ade2::hisG | This study |
| REV | TW13044 | CAI8 | ADE2::(proERG11[205-301] proMDR1[500]-RLUC)/ade2::hisG | This study |
| 1206 | TW04973 | CAI8 | ADE2::(proERG11[1206]-RLUC)/ade2::hisG | 30 |
| 806 | TW04975 | CAI8 | ADE2::(proERG11[806]-RLUC)/ade2::hisG | This study |
| 572 | TW04977 | CAI8 | ADE2::(proERG11[572]-RLUC)/ade2::hisG | This study |
| 419 | TW04978 | CAI8 | ADE2::(proERG11[419]-RLUC)/ade2::hisG | This study |
| 301 | TW04979 | CAI8 | ADE2::(proERG11[301]-RLUC)/ade2::hisG | This study |
| 276 | TW12905 | CAI8 | ADE2::(proERG11[276]-RLUC)/ade2::hisG | This study |
| 276-Ab | TW14054 | CAI8 | ADE2::(proERG11[276-A]-RLUC)/ade2::hisG | This study |
| 276-Bb | TW14051 | CAI8 | ADE2::(proERG11[276-B]-RLUC)/ade2::hisG | This study |
| 276-Cb | TW14049 | CAI8 | ADE2::(proERG11[276-C]-RLUC)/ade2::hisG | This study |
| 276-Db | TW14046 | CAI8 | ADE2::(proERG11[276-D]-RLUC)/ade2::hisG | This study |
| 276-Eb | TW14063 | CAI8 | ADE2::(proERG11[276-E]-RLUC)/ade2::hisG | This study |
| 276-Fb | TW15310 | CAI8 | ADE2::(proERG11[276-F]-RLUC)/ade2::hisG | This study |
| 276-ΔFb | TW14370 | CAI8 | ADE2::(proERG11[276-ΔF]-RLUC)/ade2::hisG | This study |
| 276-2Fb | TW12942 | CAI8 | ADE2::(proERG11[276-2F]-RLUC)/ade2::hisG | This study |
| 276-Gb | TW15319 | CAI8 | ADE2::(proERG11[276-G]-RLUC)/ade2::hisG | This study |
| 246 | TW12903 | CAI8 | ADE2::(proERG11[246]-RLUC)/ade2::hisG | This study |
| 217 | TW12901 | CAI8 | ADE2::(proERG11[217]-RLUC)/ade2::hisG | This study |
| 205 | TW04980 | CAI8 | ADE2::(proERG11[205]-RLUC)/ade2::hisG | This study |
| 99 | TW04970 | CAI8 | ADE2::(proERG11[99]-RLUC)/ade2::hisG | This study |
| BWP17 | TW14901 | SC5314 | ura3::imm434/ura3::imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG | 37 |
| Δupc2/Δupc2 | TW15343 | BWP17 | upc2::URA3/upc2::ARG4 | 29 |
| BWP17 276/ADE2/SAT1 | TW13004 | BWP17 | ADE2::(SAT1/proERG11[276]-RLUC)/ade2::hisG | This study |
| Δupc2/Δupc2 276/ADE2/SAT1 | TW13007 | Δupc2/Δupc2 | ADE2::(SAT1/proERG11[276]-RLUC)/ade2::hisG | This study |
a
Relative genotype is the genetic loci that differ from those of the parental strain.
b
The ERG11 promoter sequence for these strains is shown in Fig. 5A.