FIG. 3.

Putative complex I dimers in wild-type mitochondria after solubilization with Triton X-100 or DDM. 2D BN-SDS-PAGE of crude mitochondria solubilized with Triton X-100 (A) or DDM (B) at a detergent/protein ratio of 1.5 g/g. The subunits of monomeric complex I (I₁) and putative complex I dimers (I₂) are marked by boxes. One subunit of the pyruvate dehydrogenase complex (PDC; spot 4 of Table 1) and two subunits of the NAD⁺-dependent isocitrate dehydrogenase (IDH; spots 41 and 42 of Table 1) are indicated as in Fig. 2. (C) 2D BN/BN-PAGE of crude mitochondria solubilized with Triton X-100 at a detergent/protein ratio of 1.5 g/g. The separated complex I monomers of individual complex I (I₁) and dimeric complex I (I₂) are marked by arrowheads.