FIG. 4.
In-gel activity assays of NADH dehydrogenase, cytochrome c oxidase, ATP hydrolase, and succinate dehydrogenase. BN-PAGE of digitonin-solubilized mitochondria from bovine heart (BHM) as a con-trol, N. crassa wild type (wt), and eight complex I deletion mutants (nuo9.8, nuo11.5, nuo14, nuo20.8, nuo21, nuo29.9, nuo30.4, and nuo51). (A) NADH dehydrogenase (purple bands), reactive bands of wt and nuo21 corresponding to complex I are marked by red bars, and the red box indicates the active band of the peripheral arm of complex I from the four membrane arm mutants. (B to D) Cytochrome c oxidase (brown-yellowish bands) (B), ATP hydrolase (black-white bands) (C), and succinate dehydrogenase (purple band) (C). In all panels, some OXPHOS complexes and supercomplexes, like a to e (I1III2IV0-4) of bovine heart mitochondria, are marked on the left side.