FIG. 5.

Wild-type-like respiratory supercomplexes in peripheral arm mutants nuo21 and nuo51. The nuo51 mutant lacking the 51-kDa subunit forms stable respirasomes containing complexes I, III, and IV without NADH oxidase activity. Results shown are from 2D BN-SDS-PAGE of digitonin-solubilized crude mitochondria of nuo21 (A) and nuo51 (B). The markings are according to the legend of Fig. 1. Additionally, each one subunit of complex I (spot 12 in Table 1), complex III (spot 24 in Table 1), complex IV (spot 43 in Table 1), and complex V (spot 22 in Table 1) are indicated as described for Fig. 2.