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. 2007 Oct 19;6(12):2365–2375. doi: 10.1128/EC.00275-07

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Copyright © 2007, American Society for Microbiology

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FIG. 8. — A system for the simultaneous expression of two proteins from single-copy transgenes targeted to argB and pyroA. (A) Targeted integration of the transforming plasmids to the argB and pyroA genes, located at chromosomes III and IV, respectively. Use of the argB^Bgl2 allele for site-directed integration has been reported previously (31, 39). (B) Western blotting analysis of PalH-GFP and PalI-(HA)₃ expression driven by alcA^p. Strains carrying (+) or lacking (−) the transgenes are indicated. The PalH-GFP transgene was integrated at argB, whereas that driving expression of PalI-(HA)₃ was integrated at pyroA. The top and bottom panels were revealed with anti-GFP and anti-HA antibodies, respectively.