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. 2007 Oct 5;6(12):2214–2221. doi: 10.1128/EC.00284-06

FIG. 3.

FIG. 3.

Flo11 is a mannoprotein. A slot blot is presented, showing purified Flo11 protein applied to a PVDF membrane and treated with a digoxigenin-labeled plant lectin that specifically interacts with terminal mannose residues (GNA). Each lane contains three replicates of a protein sample. Lane 1, nickel column-purified Flo11p from S. cerevisiae var. diastaticus strain YeAD5 transformed with pFLO11-GPIΔ; lane 2, column-purified Flo11p from Σ1278b strain L5487 flo11Δ transformed with pFLO11-GPIΔ; lane 3, fetuin (negative control); lane 4, transferrin (negative control); lane 5, carboxypeptidase Y (positive control).