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. 2007 Oct 5;6(12):2214–2221. doi: 10.1128/EC.00284-06

TABLE 1.

Yeast strains and plasmids used in this study

Yeast strain or plasmid Strain background Genotype or description Reference or source
Strains
    L5487 Σ1278b MATα leu2::hisG ura3-52 37
    L5487 flo11Δ Σ1278b MATα leu2::hisG ura3-52 flo11::URA3 29
    L5489 Σ1278b MATa/α leu2::hisG/leu2::hisG ura3-52/ura3-52 C. Styles and G. Fink
    YIY345 S. cerevisiae var. diastaticus MATaura3 leu2-3,112 his4 sta0 47
    YIY345 flo-1 S. cerevisiae var. diastaticus MATaura3 leu2-3,112 his4 sta0flo11::URA3 30
    YeAD5 S. cerevisiae var. diastaticus MATα leu2-3,112 ura3-Δ227 arg4 STA1 30
    M1800D × YIY319 S. cerevisiae var. diastaticus MATa/α leu2-3,112/LEU2 arg4/ARG4 ura3/URA3 6
    FY86 S288C MATα his3Δ200 ura3-52 leu2Δ1 46
Plasmids
    pFLO11 YEplac181-PGK1p-MUC1 8
    pFLO11-GPIΔ YEplac181-PGK1p-MUC1-GPIΔ This work; derived from YEplac181-PGK1p-MUC1 (8)