FIG. 2.
In vivo O mannosylation in the ΔAfpmt1 mutant. Two hundred micrograms of AfCHI44 purified from the wild-type or ΔAfpmt1 mutant strain was used in each assay as described in Materials and Methods. The O-glycans were analyzed with a Carbo-PA1 column on an HPAEC-PAD. The glycans were eluted with 15 mM NaOH at a flow rate of 1.0 ml/min. The mannose standard used for HPAEC-PAD analysis was treated by β-elimination before injection, and thus the peaks marked “Man” indicate the retention times of anhydrous mannitol, which is derived from mannose treated by β-elimination reaction with NaBH4. This experiment was repeated at least three times, and representative data are shown.