FIG. 3.

Temperature-sensitive and osmotically stabilized phenotypes of ΔAfpmt1 mutant. A series of 10-fold dilutions (10⁷ to 10³ cells) of YJ-407, ΔAfpmt1, revertant, and CEA17 strains of A. fumigatus were cultivated on solid complete medium at 37°C, 42°C, or 50°C for 16 to 18 h. The wild-type strain YJ-407 (Afpmt⁺ pyrG⁺) and the parental strain of the mutant, CEA17 (Afpmt⁺ pyrG⁻), were set as controls for the ΔAfpmt1 mutant (Afpmt⁻ pyrG⁺) and the revertant (Afpmt⁺ pyrG⁻), respectively.